A Study on N-[4-hydroxyphenyl] Retinamide (4-HPR) Increasing the Radiotherapy Sensitivity on Bladder Cancer Cell Line
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摘要: 目的: 观察N-(4-羟苯基)维甲酰胺(4-HPR)联合γ-射线对人膀胱移行细胞癌的生长抑制和诱导凋亡作用,分析两者之间是否存在协同作用. 方法: 应用四甲基偶氮蓝比色(MTT)法检测4-HPR对膀胱癌细胞株T24的细胞毒性作用、半数抑瘤率及起效浓度和时间.应用流式细胞仪检测4-HPR、γ-射线及两者联合应用时对T24细胞生长抑制作用. 结果: 2.5μmol/L、5μmol/L、10μmol/L的4-HPR作用T24细胞5天的凋亡率分别为6.2%、28.0%和26.0%,死亡率分别为0.3%、12.5%和26.7%.联合作用组细胞分别加入2.5μmol/L、5μmol/L的4-HPR,100cgy的γ-射线照射一次,3天后T24细胞的凋亡率分别为20.0%、26.0%,细胞的死亡率均在5.0%以下.5天后T24细胞的凋亡率分别为38.0%、35.0%,而细胞的死亡率分别为12.4%、21.1%. 结论: 小剂量的4-HPR可以诱导T24细胞凋亡,大剂量的4-HPR主要导致T24细胞死亡.小剂量的4-HPR联合小剂量的γ-射线对T24细胞凋亡有明显的协同作用.Abstract: Objective :To study the growth suppression and induced apoptosis combined effect of 4-HPR and y-radiation on transitional cancer cells of human bladder and to analyze whether there would be synergistic action between them. Methods :MTT calorimetric assay was employed to examine the growth status of the cells. Flow cytometer was employed to detect the growth inhibition effect of 4HPR and/or y-radiation on bladder cancer cell line T24. Results :te were 0.3%, 12.5% and 26.7%, respectively. In the group of treatment with 2.SN.,mo1/L or SN.,mol/L 4-HPR and 100cgy y-radiation, on day 3 the apoptosis ratio were 20.0% and 26.0%, and the average mortality rate of cells were below 5.0%; whereas on day 5 the apoptosis ratio were 38.0% and 35.0%, and the mortality rate were 12.4% and 21.1% respectively. Conclusions :The apoptosis ratio of 2.SN.,mo1/L,SN.,mol/L and lON.,mol/L 4-HPR-[reated T24 cells on day 5 were 6.2%,28.0% and 26.0%,and the mortality raConclusion: Micro-dosage 4-HPR may induce T24 cells apoptosis, where maximus dosage 4-HPR may induce cell death. Micro-dosage 4-HPR combined with Micro-dosage γ-radiation have the synergistic action on T24 cells apoptosis.
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Key words:
- 4-HPR /
- Radiosensitivity /
- Bladder cancer /
- Apo ptosls
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